Eatfrenchfries Posted November 2, 2023 Share Posted November 2, 2023 (edited) Starting to get my next batch of PNSB ready for the next meet. Looking to get a head count to see how much culture media needs to be prepared. Batches take 20 - 30 days for proper incubation. PNSB -food for bacterivores (inverts,corals) + rich in carotenoids and B vitamins -water clarifier (reduce organics) -nutrient export (Waste consumed can be recycled as food for filter feed or exported via skim/floss) -bacterial competitor (competes with vibrio, cyano, algae, and dinos for space) caption: 16oz bottle of PNSB caption: 1 Gallon size Species: confirmed Rhodopseudomonas palustris , suspected Rhodospirillum rubrum and Rhodobacter sphaeroides Location: Raleigh Hills, OR Price: $1 per fluid ounce / *$100 for 1 gallon (128oz)* Dosage: 1mL treats 5 gallons, 16oz treats 2,365 gallons, 1 gallon treats 18,920 gallons Preorder ends: 11/12 Bacteria available for pickup: starting 12/10 (meetup at Holiday party?) Edited November 2, 2023 by Eatfrenchfries added dosage 2 Link to comment Share on other sites More sharing options...
islandVib3s Posted November 3, 2023 Share Posted November 3, 2023 1 hour ago, Eatfrenchfries said: Starting to get my next batch of PNSB ready for the next meet. Looking to get a head count to see how much culture media needs to be prepared. Batches take 20 - 30 days for proper incubation. PNSB -food for bacterivores (inverts,corals) + rich in carotenoids and B vitamins -water clarifier (reduce organics) -nutrient export (Waste consumed can be recycled as food for filter feed or exported via skim/floss) -bacterial competitor (competes with vibrio, cyano, algae, and dinos for space) caption: 16oz bottle of PNSB caption: 1 Gallon size Species: confirmed Rhodopseudomonas palustris , suspected Rhodospirillum rubrum and Rhodobacter sphaeroides Location: Raleigh Hills, OR Price: $1 per fluid ounce / *$100 for 1 gallon (128oz)* Dosage: 1mL treats 5 gallons, 16oz treats 2,365 gallons, 1 gallon treats 18,920 gallons Preorder ends: 11/12 Bacteria available for pickup: starting 12/10 (meetup at Holiday party?) Put me down for a gallon. This is the way! 1 Link to comment Share on other sites More sharing options...
NanoNerd Posted November 3, 2023 Share Posted November 3, 2023 Yesssss! I'd be thrilled to get my greedy hands on some purple bacteria friends! We'd love to pre-order 16oz! 💜 1 Link to comment Share on other sites More sharing options...
obrien.david.j Posted November 3, 2023 Share Posted November 3, 2023 I'll need another 32oz by then please. BTW, I'll bring my current two 32oz bottles back to you, empty. 1 Link to comment Share on other sites More sharing options...
Confused_reefer Posted November 3, 2023 Share Posted November 3, 2023 Can this be refrigerated/stored? For how long? I'd love to grab a gallon if it can be stored. 1 Link to comment Share on other sites More sharing options...
half-astronaut Posted November 3, 2023 Share Posted November 3, 2023 I’ll take 32oz 1 Link to comment Share on other sites More sharing options...
Jeff.Tichenor Posted November 3, 2023 Share Posted November 3, 2023 I'll take 32oz 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted November 3, 2023 Author Share Posted November 3, 2023 1 hour ago, Confused_reefer said: Can this be refrigerated/stored? For how long? I'd love to grab a gallon if it can be stored. It is best stored at room temperature in a spot that does not get too much light. It will continue to "grow" this way. It can be refrigerated to prolong its shelf life. The colder temperature will stop any growth/decay. Shelf life: room temp- up to 1 year from date packaged. Possibly longer if kept in ideal conditions. *bacteria products stored in the fridge tend to act a bit slower since they seem to get themselves back to health before resuming function* 1 Link to comment Share on other sites More sharing options...
half-astronaut Posted November 4, 2023 Share Posted November 4, 2023 Btw, that Dremel-fuge is pretty MacGuyver. Well played. 1 Link to comment Share on other sites More sharing options...
Jeff.Tichenor Posted November 5, 2023 Share Posted November 5, 2023 @Eatfrenchfries How often should we dose PNSB? Quote Dosage: 1mL treats 5 gallons, 16oz treats 2,365 gallons, 1 gallon treats 18,920 gallons Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted November 5, 2023 Author Share Posted November 5, 2023 3 hours ago, Jeff.Tichenor said: @Eatfrenchfries How often should we dose PNSB? Depends on what your agenda is. For feeding I would suggest daily. For colonizing your tank: large dose weekly (2-5x usual dose) skimmer off, uv off. Gutloading: soak fish food in pnsb whenever you'd like to give your fish a boost (probiotic) Treating nuisances: dose every 3 days. *Consistency is key above all* Personally, I feed my zooplanktom cultures PNSB daily. Once a week I add 10floz per 5gallons to keep things clean (usually after my phyto water change). For a reef I alternate phyto and pnsb each day. If I am trying to move nutrients then I dose with the skimmer off for during day and on at night. Skimmer will work harder during this time. 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted November 5, 2023 Author Share Posted November 5, 2023 Sweet spot from feedback: dosing every other day. <*food for thought: once PNSB is established in your tank it should produce provided it has the necessary reactants. The limiting factor would be the type of organics your system has to keep the PNSB blooming. Possibly adding additional PNS Bacteria is not beneficial but rather the nutrient media it comes with is. Issue is the nutrient media is an excellent fertilizer for other microbes if not already spoken for. Even other bacteria products in the field could be contributing more from the nutrients it adds rather than the bacteria themselves once "seeded"> 1 Link to comment Share on other sites More sharing options...
Cholland Posted November 6, 2023 Share Posted November 6, 2023 I’d like to pre order 16oz please. 1 Link to comment Share on other sites More sharing options...
don_chuwish Posted November 9, 2023 Share Posted November 9, 2023 I'd take 32oz. 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted November 10, 2023 Author Share Posted November 10, 2023 Last chance to get in on the pre-order! After today I cannot guarantee any availability. Link to comment Share on other sites More sharing options...
LavenderTi Posted November 11, 2023 Share Posted November 11, 2023 I’ll do 16 oz 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 7, 2023 Author Share Posted December 7, 2023 (edited) Just had a smelly harvest. Pickup is this Sunday at the holiday party. If you will not be attending please let me know so we can work something out. I have approximately 1.3 gallons (5000mL) of PNSB up for grabs. *next batch is scheduled for January 8th picture: sea purslane grown at 1.025 in my purple bacteria/mangrove tank Edited December 7, 2023 by Eatfrenchfries Eye candy 2 Link to comment Share on other sites More sharing options...
Jeff.Tichenor Posted December 7, 2023 Share Posted December 7, 2023 Love those bags!! 1 Link to comment Share on other sites More sharing options...
half-astronaut Posted December 8, 2023 Share Posted December 8, 2023 I vote for a meeting at @Eatfrenchfries house/lab. I bet he’s got a ton of sh*t he’s not advertising that would be fascinating. 1 2 Link to comment Share on other sites More sharing options...
Tiny Reef Posted December 9, 2023 Share Posted December 9, 2023 I'll take 16oz if it's still available. I'll be at the holiday party. 1 Link to comment Share on other sites More sharing options...
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