I started with dead sand (mostly- seeded with a few cups from my old aquarium) and live rock. I let sit for a couple of weeks, expecting a nitrate spike but never had one- so I figured it was safe to proceed (slowly). I started with 6 green chromis (which I now regret!), and a small clean-up crew and slowly added the stuff from my old tank over a month or so. Never had cyano, diatoms, algae (still don't- frantically knocking on wood right now).