Excess Phyto (blend) + Pod (blend) available

[Eatfrenchfries]

Finished harvesting phyto from the new reactors. Had enough excess to make 6 bottles of blend. 

Isochrysis + Tetraselmis + Nannochloropsis + Thalassiosira.

There's a splash of Pyrocystis and Porphyridium as well. 

 

Located in Portland, $10 for 16oz bottle. Cheaper in bulk. Shipping available 

A few flasks of nanno + iso are available well.

Edited January 9 by Eatfrenchfries

[Eatfrenchfries]

White worms available as well. Gutloaded with yogurt + selcon

[Eatfrenchfries]

Single strains for master culture.

[Eatfrenchfries]

Handful of caulerpa lentillifera mixed with shreds of gracilaria parvispora. Nutrient export or tang food. $5

[Eatfrenchfries]

Let me know if you need phyto before I go on holiday 12/24 - 12/31. Made plenty to keep up with requests. Felt bad for running out last time 

Shipping available USPS. 

[John Vinson]

PM sent

[Eatfrenchfries]

Rotifers available. $10 for 16oz bottle. Bring your own 1/2 gallon container and I'll fill it for $20. 

 

L type available. 

S type coming soon.....

 

 

 

[LadAShark]

On 12/20/2022 at 4:21 PM, Eatfrenchfries said:

Rotifers available. $10 for 16oz bottle. Bring your own 1/2 gallon container and I'll fill it for $20. 

 

L type available. 

S type coming soon.....

 

 

 

Ooh, wish I saw this when I got the phyto from you. Can those rotifers survive/breed in the reef? Would be great if I could get some that can colonize my tanks. 

[Eatfrenchfries]

They can if you have no mechanical filtration and pads + socks. It's much better practice to keep the rotifers reproducing in a separate container and just harvest/feed accordingly. 

[Eatfrenchfries]

Back from holiday. Had a few mishaps keeping the cultures automated for the first time but thankfully there's always master cultures. Picture included below of the 500mL strains I keep 24/7 in case of crashes. Also keep 5mL of cells to start over again as well. 

 

Phyto, pods, and rotifers are good to go. Phyto this week is a bit more Iso concentrated since that's my surplus until my next harvest 1/9. 

 

 

Picture is of one lone black mangrove next to a bundle of reds zip tied together. 

 

[Eatfrenchfries]

Harvesting the next batch of phyto tomorrow night so get your orders in now! Copepod density has increased enough to produce a few bottles each month. Currently have a blend of tig, tisbe, apocyclops, and parvo. 

$15 pods 

$10 for phyto blend ( still running ISO dominant until nanno quantities are back up to equal.)

Located in Portland, nearest crossroad is Oleson Rd x Scholls Ferry.

 

 

Also pictured is some Rhodopseudomonas palustris (purple non sulphuric bacteria) fed algae exudates + a couple other goodies. Just cleared the 30 day incubation period + 15 day gutloading. Time to test it out. Currently playing around with nutrient recycling + NPS food for dendronephthya. 

Edited January 24 by Eatfrenchfries

[Eatfrenchfries]

Current density is around ~8million cells per mL for the ISO with the custom F/2 formula. Baking soda is key. 

[obrien.david.j]

10 hours ago, Eatfrenchfries said:

Current density is around ~8million cells per mL for the ISO with the custom F/2 formula. Baking soda is key. 

Image is broken.  repost please.     

What method do you use to measure culture density.   Can you show a picture of what you use, and how you do it?

And what's the magic of baking soda.   Interested.

[Eatfrenchfries]

@obrien.david.j

Currently using a secchi stick for rough estimates on culture density. Every so often I'll use my microscope to try and do an actual cell count. Still trying to find a mount to fit around the eyepiece for my phone.  Basically get a tally counter and count the amount in the slide and multiply. 

 

Insert secchi stick into water and when the target mark disappears you read the depth. There's a conversion chart for different phyto strains on how dense they can be. 

 

Baking soda is used for Alk, balancing pH, and adjusting CO2 levels (important if you do spirulna or motile algaes). Used in the standard Guillard F/2 but not in the right quantity to culture a few strains of the other phyto effectively. Started to mix my own F/2 with great success that I've been able to slowly tune to each strain I add. Basically you're keeping production going even during lights off on your culture with it. Not saying this is Iso specific or the key but it's one of the steps to keeping it going. 

 

Picture is of secchi stick inserted into one of my Isochrysis cultures. 

Edited January 9 by Eatfrenchfries

[obrien.david.j]

Thanks.   Interesting.  Just ordered a secchi stick to play with.   

[Eatfrenchfries]

Too bad I can't post videos. It's really cool watching the cells flit about.  This is what it looks like under a microscope. 

[Eatfrenchfries]

Sold out of pods for this harvest. Should have more in 10 days. Still got plenty of phyto to go around though. 

 

 

[Mooseman_Sal]

51 minutes ago, Eatfrenchfries said:

Sold out of pods for this harvest. Should have more in 10 days. Still got plenty of phyto to go around though. 

 

 

I'm intrested in buying your phyto and pods 

[Eatfrenchfries]

Please DM

[Mooseman_Sal]

6 hours ago, Eatfrenchfries said:

Please DM

Says your inbox is full

[Eatfrenchfries]

@Mooseman_Sal just cleared my inbox. Also tried to send you a DM but unable to.