Eatfrenchfries Posted December 15, 2022 Share Posted December 15, 2022 (edited) Finished harvesting phyto from the new reactors. Had enough excess to make 6 bottles of blend. Isochrysis + Tetraselmis + Nannochloropsis + Thalassiosira. There's a splash of Pyrocystis and Porphyridium as well. Located in Portland, $10 for 16oz bottle. Cheaper in bulk. Shipping available A few flasks of nanno + iso are available well. Edited January 9, 2023 by Eatfrenchfries Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 15, 2022 Author Share Posted December 15, 2022 White worms available as well. Gutloaded with yogurt + selcon Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 16, 2022 Author Share Posted December 16, 2022 Single strains for master culture. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 16, 2022 Author Share Posted December 16, 2022 Handful of caulerpa lentillifera mixed with shreds of gracilaria parvispora. Nutrient export or tang food. $5 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 20, 2022 Author Share Posted December 20, 2022 Let me know if you need phyto before I go on holiday 12/24 - 12/31. Made plenty to keep up with requests. Felt bad for running out last time Shipping available USPS. Link to comment Share on other sites More sharing options...
John Vinson Posted December 20, 2022 Share Posted December 20, 2022 PM sent Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 21, 2022 Author Share Posted December 21, 2022 Rotifers available. $10 for 16oz bottle. Bring your own 1/2 gallon container and I'll fill it for $20. L type available. S type coming soon..... Link to comment Share on other sites More sharing options...
LadAShark Posted December 29, 2022 Share Posted December 29, 2022 On 12/20/2022 at 4:21 PM, Eatfrenchfries said: Rotifers available. $10 for 16oz bottle. Bring your own 1/2 gallon container and I'll fill it for $20. L type available. S type coming soon..... Ooh, wish I saw this when I got the phyto from you. Can those rotifers survive/breed in the reef? Would be great if I could get some that can colonize my tanks. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted December 30, 2022 Author Share Posted December 30, 2022 They can if you have no mechanical filtration and pads + socks. It's much better practice to keep the rotifers reproducing in a separate container and just harvest/feed accordingly. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 3, 2023 Author Share Posted January 3, 2023 Back from holiday. Had a few mishaps keeping the cultures automated for the first time but thankfully there's always master cultures. Picture included below of the 500mL strains I keep 24/7 in case of crashes. Also keep 5mL of cells to start over again as well. Phyto, pods, and rotifers are good to go. Phyto this week is a bit more Iso concentrated since that's my surplus until my next harvest 1/9. Picture is of one lone black mangrove next to a bundle of reds zip tied together. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 9, 2023 Author Share Posted January 9, 2023 (edited) Harvesting the next batch of phyto tomorrow night so get your orders in now! Copepod density has increased enough to produce a few bottles each month. Currently have a blend of tig, tisbe, apocyclops, and parvo. $15 pods $10 for phyto blend ( still running ISO dominant until nanno quantities are back up to equal.) Located in Portland, nearest crossroad is Oleson Rd x Scholls Ferry. Also pictured is some Rhodopseudomonas palustris (purple non sulphuric bacteria) fed algae exudates + a couple other goodies. Just cleared the 30 day incubation period + 15 day gutloading. Time to test it out. Currently playing around with nutrient recycling + NPS food for dendronephthya. Edited January 24, 2023 by Eatfrenchfries Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 9, 2023 Author Share Posted January 9, 2023 Current density is around ~8million cells per mL for the ISO with the custom F/2 formula. Baking soda is key. Link to comment Share on other sites More sharing options...
obrien.david.j Posted January 9, 2023 Share Posted January 9, 2023 10 hours ago, Eatfrenchfries said: Current density is around ~8million cells per mL for the ISO with the custom F/2 formula. Baking soda is key. Image is broken. repost please. What method do you use to measure culture density. Can you show a picture of what you use, and how you do it? And what's the magic of baking soda. Interested. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 9, 2023 Author Share Posted January 9, 2023 (edited) @obrien.david.j Currently using a secchi stick for rough estimates on culture density. Every so often I'll use my microscope to try and do an actual cell count. Still trying to find a mount to fit around the eyepiece for my phone. Basically get a tally counter and count the amount in the slide and multiply. Insert secchi stick into water and when the target mark disappears you read the depth. There's a conversion chart for different phyto strains on how dense they can be. Baking soda is used for Alk, balancing pH, and adjusting CO2 levels (important if you do spirulna or motile algaes). Used in the standard Guillard F/2 but not in the right quantity to culture a few strains of the other phyto effectively. Started to mix my own F/2 with great success that I've been able to slowly tune to each strain I add. Basically you're keeping production going even during lights off on your culture with it. Not saying this is Iso specific or the key but it's one of the steps to keeping it going. Picture is of secchi stick inserted into one of my Isochrysis cultures. Edited January 9, 2023 by Eatfrenchfries Link to comment Share on other sites More sharing options...
obrien.david.j Posted January 9, 2023 Share Posted January 9, 2023 Thanks. Interesting. Just ordered a secchi stick to play with. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 10, 2023 Author Share Posted January 10, 2023 Too bad I can't post videos. It's really cool watching the cells flit about. This is what it looks like under a microscope. 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 23, 2023 Author Share Posted January 23, 2023 Sold out of pods for this harvest. Should have more in 10 days. Still got plenty of phyto to go around though. 1 Link to comment Share on other sites More sharing options...
Mooseman_Sal Posted January 23, 2023 Share Posted January 23, 2023 51 minutes ago, Eatfrenchfries said: Sold out of pods for this harvest. Should have more in 10 days. Still got plenty of phyto to go around though. I'm intrested in buying your phyto and pods 1 Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 23, 2023 Author Share Posted January 23, 2023 Please DM Link to comment Share on other sites More sharing options...
Mooseman_Sal Posted January 24, 2023 Share Posted January 24, 2023 6 hours ago, Eatfrenchfries said: Please DM Says your inbox is full Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted January 24, 2023 Author Share Posted January 24, 2023 @Mooseman_Sal just cleared my inbox. Also tried to send you a DM but unable to. Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted May 8, 2023 Author Share Posted May 8, 2023 (edited) 5 - 7 - 2023 Phytoplankton Blend (4) - $8 for 16oz / $60 for 128 oz -(Nanno, Iso, Tet, Thal) Phytoplankton Blend (7) - $12 for 16oz / $85 for 128oz -(Nanno, Iso, Tet, Thal, Por, Rhodo, Sym) *DM for inquiry into single strain pricing/availability* Copepod Blend (4) - $15 for 16oz -(Tig, Tis, Apo, Parvo) L Rotifers - $10 for 16oz Shipping: $15 to ship orders under $100, orders over $100 are free in OR/WA. Local Pickup: Raleigh Hills More Info Here: Highlight001_1.mp4 Edited May 8, 2023 by Eatfrenchfries 1 1 Link to comment Share on other sites More sharing options...
freddyk Posted May 8, 2023 Share Posted May 8, 2023 PM Sent Link to comment Share on other sites More sharing options...
Eatfrenchfries Posted June 19, 2023 Author Share Posted June 19, 2023 Wholesale phytoplankton blend *contact directly for pricing Minimum 5gallons. (Maximum 30g weekly) Location: Portland 2 Link to comment Share on other sites More sharing options...
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