EMeyer Posted October 15, 2019 Share Posted October 15, 2019 (edited) I think we've all been there. What do I do? It had two branches and was STNing from the base. I cut the branches, made two frags, and hoped for the best. A month or so went by. I learned some things about the microbiome of reef tanks, thanks in part to the samples many of you contributed. During my analysis and reading I came up with a crazy idea for a treatment for tissue necrosis (STN and RTN). Soon enough, my two little branches started to STN again. I treated one frag with my experimental treatment and did nothing to the other. I watched, and waited. Here's a photo I took tonight of the two frags -- untreated on the left, treated on the right. Can you spot the difference? I hope you'll agree this evidence is interesting despite the lack of statistical tests or p-values. Yes, its a n of 1. But n always starts with 1... Wouldnt it be nice, when a frag starts to RTN or STN, if you could do something about it instead of watching and hoping? On that specific detail, stay tuned. More broadly -- this is the tip of the iceberg for potential applications to understanding the microbiome of our aquariums. Thanks again for everyone who has contributed samples. And for anyone still holding a sampling kit, please do send it in so we can learn more! AquaBiomics - microbiome testing for aquariums and aquaculture Article: What kind of microbes grow in a reef tank? Edited October 15, 2019 by EMeyer 4 Quote Link to comment Share on other sites More sharing options...
albertareef Posted October 15, 2019 Share Posted October 15, 2019 Excited to hear what you tried and whether or not it's repeatable and transferable. Quote Link to comment Share on other sites More sharing options...
EMeyer Posted October 15, 2019 Author Share Posted October 15, 2019 The moment I can, I will share more details. What I can say is that in my testing of an experimental treatment I have treated several frags of several species that showed RTN or STN. So far I have not lost a single treated frag. But most of those lack untreated controls, because most of my frags are things I want to keep I am working on inducing RTN in disposable frags for a controlled and replicated experiment. Safety? I am absolutely convinced, I've treated many frags now. Efficacy? My n is still 1 for this so I am not making any claims other than "this is interesting..." I really am not a snake oil guy. But when I find a compound thats been published in peer reviewed journals, used for decades in a diagnostic capacity, and it shows promising results in my early trials... I start to think there may be something here 2 Quote Link to comment Share on other sites More sharing options...
Trailermann Posted October 15, 2019 Share Posted October 15, 2019 OK, buddy. I will pull the kit off the shelf and process it and mail it in. Nice to know that my single simple sample will be contributing to your solid scientific studies. (Onomatopoeia over use?.) Quote Link to comment Share on other sites More sharing options...
obrien.david.j Posted October 16, 2019 Share Posted October 16, 2019 Eli, I had some STN going on in my tank when I submitted the latest sample. Noted in the submission text. In case you’re trying to connect STN to your microbiome analysis. (userid daveo) Quote Link to comment Share on other sites More sharing options...
EMeyer Posted October 16, 2019 Author Share Posted October 16, 2019 Thats great... err I mean I am sorry to hear that. Please see PM Quote Link to comment Share on other sites More sharing options...
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