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EMeyer

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EMeyer last won the day on January 29

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About EMeyer

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    Monroe, OR

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  1. EMeyer

    UV LED flashlights

    Really interesting, thanks for the photos. That definitely looks like the flashlight is exciting coral FPs. Either the flashlight has a spectrum that bleeds over into the excitation wavelengths of the proteins, or the proteins have broader excitation peaks in the living animal than they do as purified proteins in a tube... Looking more closely at the spectra from the paper I linked, many of them do have a small secondary excitation peak in the UV range... so maybe not so unexpected after all. Blue should still produce brighter fluorescence, but apparently UV does enough to put on a show too
  2. <1% of marine microbes can be cultured. Focusing on culturable species only is incredibly limiting, which is why modern research has stopped focusing on them and now uses DNA sequencing to study all the bugs instead. Obviously there is huge value in working with the culturable strains, for functional studies, but the huge and growing field of microbiomics now uses DNA sequencing for most work. Here are some of my questions: Does adding bacteria in a bottle actually change the microbiome of your tank? Does starting with live rock lead to a more diverse microbiome? How do commonly used aquarium supplements affect the microbiome? Could probiotics (bacterial food) be used to deliberately increase microbial diversity? Instead of asking whether my new tank is cycled in an indirect way, by measuring nutrient levels with terribly inaccurate hobbyist kits, can we use direct measurements of the microbiome? And I share the interest in the question already brought up -- how does my tank's microbiome compare to that of a wildly successful SPS reef tank? What else would people be curious to ask using this technology, if it were to suddenly one day become available for the aquarium hobby?
  3. EMeyer

    UV LED flashlights

    Fluorescence in UV is probably mostly coming from other sources than coral's fluorescent proteins, which is where corals get nearly all of their color. Photosynthetic and accessory pigments are excited in that range. So are a lot of other fluorescent small molecules. So I guess its not surprising something would fluoresce, out of all the animal, protist, and bacterial cells in a coral... I'm curious now, gonna have to find a UV source and see what lights up...
  4. Good question. Nope, modern methods for studying microbial diversity don't rely on plating or culturing at all. We directly extract DNA from the sample, amplify the genetic markers with PCR, and sequence the resulting DNA millions of reads at a time. The results are not perfectly quantitative for comparing between different bugs, but very good for comparing frequency of a partciular bug between samples. And theyre not affected by culturability.
  5. Absolutely. I think both hobbyist reef tanks and coral aquaculture tanks will be interesting comparisons. Thanks, thats exactly the kind of question I'm looking for.
  6. Hi everyone, Researchers analyze the microbial communities of animals or aquariums using DNA sequencing. If you, as a hobbyist, had access to a service that could measure the microbiome in your tank: what question would you be most curious in asking, with this test? We have some serious reef keeping expertise on this forum and I'm curious to hear your perspective on applications for a test like this. Thanks for any insights you can share. -Eli
  7. EMeyer

    Frag Sale Making Room

    Hey Robert, sent a PM with my order for a mixed pack if you have anything left. just noting this here in case you missed it. Thanks!
  8. EMeyer

    UV LED flashlights

    FWIW I am not aware of any coral fluorescent proteins that are maximally excited by UV. Maximum excitation ranges from blue to green (see table on third page) https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0002680&amp;type=printable I use a blue LED flashlight with yellow goggles for this purpose. Would be curious to see what gets excited by UV.
  9. I’ve got another batch of easy starter soft corals ready for sale. These are not fancy high end corals, just an affordable way to add a bunch of color and motion to your tank. I’m selling any 6 corals for $60 (shipped) or any 12 for $100 (shipped). Just pick any 6 or 12 corals from this list and message me with your list. I take payment by Paypal, and can ship anytime in the next couple weeks at your convenience. Because these are robust soft corals I am shipping priority mail USPS (~2-3 days). If you prefer, I am happy to ship overnight for additional shipping costs. (Message me and we can figure out the additional cost for your address). Alternatively, if you'd like to meet somewhere between Corvallis and Eugene to pick them up in person, we can subtract $20 from the listed price. Corals will be shipped in insulated packaging with heat packs to keep them warm and happy on the way. I’ll refund the cost of any corals that don’t make it, if something goes wrong. (As usual, I cannot refund the cost of shipping in those cases, only the DOA animals themselves). Here are the images – in some cases I include pics of the colony so you can see what it’ll grow into. ... and here is a key with some names. A. Zoanthids, 'eagle eyes'. An old standby, this grows like a weed. Develops bright colors in high light. B. Zoanthids, 'peanut butter cups'. Fast growing, unassuming by day but shows bright orange mouths under blue lights. C. Zoanthids, 'gobstoppers'. Eye catching colors from 10 feet away. D. Zoanthids, 'armor of god'. A true bright red. F. Zoanthids, 'mohawk'. Pink speckles fading to a yellow mouth. Needs high light for best colors. H. Zoanthids, 'micro kiwis'. TINY polyps, almost as yellow as King Midas under the blue lights. J. Zoanthids, 'flame skirt'. Super bright orange skirt with a bright yellow mouth and white speckles. For someone reason I can’t get photos to do this one justice. K. Palythoas, bright green. Super bright under blue lights. L. Palythoas, teal. No special fluorescence but a cool teal blue under white lights. M. Palythoas, white-faced. Initially this wild Paly looked like nothing special but turned out to show cool white markings on the face if grown under high light. N. Pulsing Xenia. Love it, hate it, this strain pulses constantly and grows as fast as you expect. O. Tubipora, light green. Highly underrated coral. The soft coral that thinks it’s a hard coral. Fast growing but does not spread. P. Green star polyps, branching. The brightest green I’ve seen in any coral, hands down. Fast growing, I suggest confining it to a rock island of its own. A branching variety. Q. Mushroom, green striped. Does well in low light. R. Mushroom, blue spotted. Likes moderate light to develop the bright blue spots. S. Mushroom, red spotted. Likes moderate to high light develop the bright red and cyan spots. Super fast growing. T. Mushroom, green hairy. Very bright green, likes to spread. U. Kenya tree. Fast growing, no special color but adds a lot of motion as it sways in the current. V. Neon green sinularia. Almost as bright green as GSP, a must have for any soft coral tank. [edit: updated to remove a couple that have sold out. everything shown is still available] [edit 2: updating whats left in stock] [edit 3: stock update]
  10. EMeyer

    Looking for 40 breeder or 20 long

    Still looking for a 40 breeder in the Eugene - Albany area. Anyone got a spare tank taking up space in your garage? I can help you with that!
  11. A mandarin, urchin, and now 2 serpent stars who I've recently banished to the refugium!
  12. Thanks Fred! It was good meeting you and I hope the corals do well in your tank.
  13. Id like to plumb an additional tank into the frag system. I could fit either of these sizes (40b or 20l) Paying $1 per gallon, willing meet anywhere between Albany to Eugene.
  14. I'm selling off some soft corals to make room in the frag tank, and still looking for a new home for this spotted mandarin and pencil urchin. All items are for sale or trade. I'd consider trades for various invertebrates, corals, or a 40g breeder tank. #1. Spotted (aka yellow) Mandarin, female, 2" ($20). Has lived happily in one of my algal refugia for about 9 months. Too pretty a fish to live hidden in a refugium, but she fights with her sister if I put her in the DT. I have recently witnessed her eating frozen mysis. #2. Pencil urchin ($10). Had him over a year. I banished him to the refugium a while back because he kept knocking stuff over. If your tank is more urchin proof, hes a great grazer. #3. (MOSTLY SOLD OUT, INQUIRE ABOUT AVAILABILITY IF STILL INTERESTED). Easy soft coral starter packs. I've added some additional zoas to the list (and if anyone has names for the ones I'm listing as unnamed, I'd be curious to hear them). I can ship these: 6 for $50 or 12 for $80 (shipped prices). Want to add color and motion to your tank for a low price? I'm trying to clear out a bunch of soft corals to make room in the frag tank. These are nothing high-end but they are big and colorful and cheap. These are great beginner soft corals, easy to keep alive and fast growing. These thrive in a wide variety of light, flow, and nutrient levels. You pick 6 or 12 from my list of soft corals. These are big, fat, well-healed soft coral frags mounted on tiles, frag plugs, or live rock rubble. The list: A. Eagle eye zoanthids. Bright green and red under blue light. B. Peanut butter cup zoanthids. Two tone browns in daylight, bright orange centers under blue light. C. Gobstopper zoanthids. Bright pink center with a bright green mouth. Very bright under blue lights. D. Neon green sinularia. Super bright green in daylight or blue lights. Grows large with lots of branches. E. Bright green palythoas. White striped pattern in center, very bright green under blue lights. F. Pale blue palythoas. Teal to light blue, very distinct from the green ones. G. Pulsing Xenia. Some love it for its constant motion and rapid growth. Some consider it a pest for the same reason. H. Tubipora. A cool mint green color. Fast growing but doesnt spread, just makes a giant bush. I. Green star polyps. This is a branching morph. Very fast growing and very bright green. J. Spotted green mushrooms. Stripes and spots in shades of green. L. Kenya tree. Big, brown, and fast growing. M: 'armor of god' zoanthids, bright red under both white lights and blues. N: unnamed zoanthid, I call it yellow and gold. Two different morphs of the same type (not two types mixed together). Metallic coppery under whites, bright yellow and gold under blues. 0: unnamed zoanthid, I call it pink and gold. Unimpressive under white lights. Under blues, it has beautiful pink flecks and a yellow mouth. Shipping: I ship these priority mail through USPS. They are robust corals that can tolerate this, and it keeps shipping costs low. I will ship each order in a styrofoam lined box with a heat pack, and will delay shipping if weather conditions are not suitable. The shipped costs listed here apply only in OR or WA. Outside of that, additional shipping charges will apply. If youd like to meet somewhere in person between Corvallis - Eugene, subtract $15 from the shipped price. If you'd like to come pick them up in Monroe OR, subtract $20 from listed price. Finally the pics! First some representative pictures of frags, then mother colony photos for some of the corals, to give you a better sense what they'll grow into.
  15. Between PNWMAS and RC I have enough sales pending. Closing the sale for now. Please close the thread when you can (if we do that here)
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